{"id":24134,"date":"2025-05-20T15:32:04","date_gmt":"2025-05-20T07:32:04","guid":{"rendered":"https:\/\/www.rocker.com.tw\/?post_type=application&#038;p=24134"},"modified":"2026-03-09T11:18:27","modified_gmt":"2026-03-09T03:18:27","slug":"hplc","status":"publish","type":"application","link":"https:\/\/www.rocker.com.tw\/en\/application\/hplc\/","title":{"rendered":"HPLC"},"content":{"rendered":"<h2 style=\"text-align: center;\"><span style=\"font-size: 24px;\"><strong>HPLC Mobile Phase Filtration: <br \/>\nThe Key Step to Enhance Chromatographic Efficiency<\/strong><\/span><\/h2>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2 style=\"text-align: left;\"><span style=\"font-size: 20px;\"><strong>Table of Contents<\/strong><\/span><\/h2>\n<p>&nbsp;<\/p>\n<div style=\"background-color: #e9eef0; padding: 10px 20px;\">\n<p><a href=\"#A1\">What is HPLC?<\/a><br \/>\n<a href=\"#A2\">Why Does HPLC Mobile Phase Need Filtration?<\/a><br \/>\n<a href=\"#A3\">Why Does Filtration Improve HPLC Stability?<\/a><br \/>\n<a href=\"#A4\">How to Perform HPLC Mobile Phase Filtration?<\/a><br \/>\n<a href=\"#A5\">ROCKER Complete Solvent Purification Solution<\/a><br \/>\n<a href=\"#A6\">How to Choose a Membrane Filter?<\/a><\/p>\n<\/div>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2 style=\"text-align: left;\"><a id=\"A1\"><\/a><span style=\"font-size: 20px;\"><strong>What is HPLC?<\/strong><\/span><\/h2>\n<p>&nbsp;<\/p>\n<p>High Performance Liquid Chromatography (HPLC) is an efficient technique for separation and analysis,<br \/>\ncapable of separating mixtures and quantitatively analyzing the proportion of each component. HPLC is widely used in various scientific fields,<br \/>\nincluding pharmaceuticals, food, cosmetics, health supplements, narcotics, forensic science, and environmental matrices.<\/p>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><a id=\"A2\"><\/a><span style=\"font-size: 20px;\"><strong>Why Does HPLC Mobile Phase Need Filtration?<\/strong><\/span><\/h2>\n<p>&nbsp;<\/p>\n<p>In HPLC, the quality of the mobile phase directly affects the accuracy, reproducibility, <br \/>\nand lifespan of the instrument. Common mobile phases such as acetonitrile (ACN), methanol (MeOH), <br \/>\nor formic acid may contain impurities such as bubbles, particles, contaminants, <br \/>\nor microorganisms. These impurities can cause several negative effects on the HPLC system, <br \/>\nincluding column clogging, excessive noise and background signals, and reduced equipment lifespan.<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 16px;\"><strong>What are the consequences of not filtering the mobile phase?<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<ul style=\"list-style-type: square;\">\n<li>Column clogging: Particle buildup affects the stability of flow rate, leads to retention time shifts, <br \/>\nand may shorten the column lifespan.<\/li>\n<li>Pump damage: Particles may wear out the piston and seals, causing baseline drift and flow fluctuations.<\/li>\n<li>System clogging: May result in increased backpressure, leading to system shutdowns.<\/li>\n<li>Interference with analysis: Interaction with column materials can cause noise <br \/>\nand background elevation in chromatograms, and even ghost peaks<br \/>\n(known as pseudo peak, eigen peak, induced peaks).<\/li>\n<li>Microbial contamination: Aqueous buffers may promote microbial growth, producing contaminants that affect results and clog the system.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>In addition, many HPLC methods involve solvent mixing and\/or the addition of salts,<br \/>\nsuch as phosphates or acetates. These extra mixing steps or additives may result in particles if not fully dissolved.<br \/>\nTherefore, filtration before use is recommended to ensure solution purity.<br \/>\nTo protect the HPLC system, extend the column&#8217;s lifespan, <br \/>\nand achieve consistent, accurate, and reproducible results, <br \/>\nmobile phase filtration is an essential and critical step.<\/p>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-size: 20px;\"><strong><a id=\"A3\"><\/a>Why Does Filtration Improve HPLC Stability?<\/strong><\/span><\/h2>\n<p>&nbsp;<\/p>\n<p>Vacuum filtration is a commonly used method for filtering mobile phases. <br \/>\nIt effectively removes particles and contaminants, preventing system blockages, reducing contamination and wear, <br \/>\nand extending the lifespan of consumables. Removing contaminants also lowers signal noise, resulting in clearer and more reliable analytical results.<br \/>\nAdditionally, vacuum filtration serves to degas the solvent, removing dissolved oxygen, <br \/>\nwhich helps minimize oxidative degradation of samples and solvents, <br \/>\nthereby improving system stability and detector sensitivity.<br \/>\nIn short, filtering the mobile phase is the simplest and most economical way to maintain HPLC performance and achieve high-quality analysis.<\/p>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><strong><span style=\"font-size: 20px;\"><a id=\"A4\"><\/a>How to Perform HPLC Mobile Phase Filtration?<\/span><\/strong><\/h2>\n<p>&nbsp;<\/p>\n<p><strong>Traditionally, HPLC solvent filtration uses a glass funnel and Erlenmeyer flask, but this approach comes with the following risks:<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li>After filtration, the solvent must be transferred to a storage bottle or serum bottle, which introduces the risk of leakage and cross-contamination.<\/li>\n<li>The solvent must be manually lifted and poured into the funnel, which can easily result in spillage due to unstable hand movement or pouring angle.<br \/>\nFor volatile or toxic solvents, <br \/>\nvapors may directly contact the operator\u2019s eyes, mouth, or nose, posing health risks with long-term exposure.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><strong><span style=\"font-size: 20px;\"><a id=\"A5\"><\/a>ROCKER Complete Solvent Purification Solution<\/span><\/strong><\/h2>\n<h2><span style=\"font-size: 16px;\">Newly launched continuous filtration device \u2013 designed to meet your large-volume solvent filtration needs<\/span><\/h2>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 20px;\"><strong>Rocker 300C &#8211; VFC 47<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-22051\" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2024\/12\/Rocker300C_VFC47Hoop_1-scaled.jpg\" alt=\"Rocker 300C - VFC 47 Continuous Solvent Purification System\" width=\"286\" height=\"279\" srcset=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2024\/12\/Rocker300C_VFC47Hoop_1-scaled.jpg 2560w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2024\/12\/Rocker300C_VFC47Hoop_1-768x750.jpg 768w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2024\/12\/Rocker300C_VFC47Hoop_1-1536x1501.jpg 1536w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2024\/12\/Rocker300C_VFC47Hoop_1-2048x2001.jpg 2048w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2024\/12\/Rocker300C_VFC47Hoop_1-600x586.jpg 600w\" sizes=\"(max-width: 286px) 100vw, 286px\" \/><\/p>\n<p>&nbsp;<\/p>\n<ul style=\"list-style-type: square;\">\n<li>Continuous feeding with direct storage in serum bottles, eliminating the need for manual transfer and avoiding cross-contamination<\/li>\n<li>Comes standard with a sinker, allowing for efficient liquid suction and reduced residue<\/li>\n<li>Equipped with GL45 filtration adapter, allowing direct collection of filtrate into GL45 serum bottles, avoiding repackaging and secondary contamination<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<table class=\" aligncenter\" style=\"border-collapse: collapse;\">\n<tbody>\n<tr>\n<td style=\"width: 25%;\"><strong>Model<\/strong><\/td>\n<td style=\"width: 25%;\">Other Traditional Bottle Sets<\/td>\n<td style=\"width: 25%;\">Rocker 300C &#8211; <br \/>\nVF 12<\/td>\n<td style=\"width: 25%;\">Rocker 300C &#8211; <br \/>\nVFC 47<br \/>\n(NEW)<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 25%;\"><strong>Product Image<\/strong><\/td>\n<td style=\"width: 25%;\"><img decoding=\"async\" src=\"https:\/\/www.rocker.com.tw\/\/wp-content\/uploads\/2025\/05\/\u4ed6\u724c\u50b3\u7d71\u74f6\u7d44.jpg\" alt=\"\" width=\"650\" height=\"650\" \/><\/td>\n<td style=\"width: 25%;\"><img decoding=\"async\" class=\"alignnone wp-image-14304 \" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2021\/02\/Rocker300C_VF12\u589e\u52a0\u652f\u67b6-2-e1747359605301.jpg\" alt=\"Rocker300C_VF12 solvent purification system with HOOP spill-proof bottle holder\" width=\"176\" height=\"194\" \/><\/td>\n<td style=\"width: 25%;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-22053 \" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2024\/12\/Rocker300C_VFC47Hoop-scaled-e1747359666772.jpg\" alt=\"\" width=\"188\" height=\"185\" \/><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 25%;\"><strong>Contact Material<\/strong><\/td>\n<td style=\"width: 25%;\">Borosilicate Glass<\/td>\n<td style=\"width: 25%;\">Borosilicate Glass<\/td>\n<td style=\"width: 25%;\">Borosilicate Glass<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 25%;\"><strong>Vacuum Pump<\/strong><\/td>\n<td style=\"width: 25%;\">Optional<\/td>\n<td style=\"width: 25%;\">PTFE Coated Vacuum Pump<\/td>\n<td style=\"width: 25%;\">PTFE Coated Vacuum Pump<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 25%;\"><strong>Direct Filtration into Serum Bottle<\/strong><\/td>\n<td style=\"width: 25%;\">X<\/td>\n<td style=\"width: 25%;\">V<\/td>\n<td style=\"width: 25%;\">V<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 25%;\"><strong>Filtration Method<\/strong><\/td>\n<td style=\"width: 25%;\">Manual Feeding<\/td>\n<td style=\"width: 25%;\">Manual Feeding<\/td>\n<td style=\"width: 25%;\">Continuous Filtration<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 25%;\"><strong>Sinker Included<\/strong><\/td>\n<td style=\"width: 25%;\">X<\/td>\n<td style=\"width: 25%;\">X<\/td>\n<td style=\"width: 25%;\">V<br \/>\n<span style=\"font-size: 12px;\">(PTFE sinker, leaves no residue)<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 25%;\"><strong>Safety &amp; Space Saving<\/strong><\/td>\n<td style=\"width: 25%;\">X<\/td>\n<td style=\"width: 25%;\">\n<p>V<\/p>\n<p><span style=\"font-size: 12px;\">(Saves 50% space)<\/span><\/p>\n<\/td>\n<td style=\"width: 25%;\">\n<p>V<\/p>\n<p><span style=\"font-size: 12px;\">(Saves 50% space)<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_29055\" aria-describedby=\"caption-attachment-29055\" style=\"width: 900px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.rocker.com.tw\/en\/contact\/\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-29055 size-full\" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2023\/04\/rocker-scientific-global-dealer-network-quick-quote-1.webp-1.webp\" alt=\"Rocker Global Dealer Network banner offering dedicated support from local experts in USA, India, China, Indonesia, Malaysia, and worldwide for quick quotes.\" width=\"900\" height=\"120\" srcset=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2023\/04\/rocker-scientific-global-dealer-network-quick-quote-1.webp-1.webp 900w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2023\/04\/rocker-scientific-global-dealer-network-quick-quote-1.webp-1-768x102.webp 768w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2023\/04\/rocker-scientific-global-dealer-network-quick-quote-1.webp-1-600x80.webp 600w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/a><figcaption id=\"caption-attachment-29055\" class=\"wp-caption-text\">Global expert support across USA, Asia, and worldwide. \u25b2Click to get a quick quote from your local Rocker dealer.<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<p><strong>The following is the system diagram of the VFC Continuous Solvent Filtration System. For details, please refer to the <a href=\"https:\/\/youtu.be\/QenMkpo88t0?feature=shared\">installation video<\/a> or see the steps below:<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li><strong>Assemble the Equipment:<\/strong><br \/>\nAttach the GL45 adaptor and VFC 47 continuous glass filter holder in sequence to the GL45 bottle.<br \/>\nConnect the silicone tubes according to the diagram.<br \/>\n<sup>* If using an Erlenmeyer flask, insert the No. 8 silicone stopper directly into the bottle mouth or replace it with a suitable size.<\/sup><\/li>\n<li><strong>Place the Membrane:<\/strong><br \/>\nPlace the membrane on top of the VFC 47 filter support, put back the glass cover, and clamp tightly with the aluminum clamp.<\/li>\n<li><strong>Start Filtration:<\/strong><br \/>\nPlace the sinker (with silicone tubing attached) into the unfiltered solution. Turn on the vacuum pump to begin filtration.<\/li>\n<li><strong>Storage:<\/strong><br \/>\nAfter filtration, remove the tubing, filter holder, and adaptor. Turn off the pump.<br \/>\nSeal the bottle with the original GL45 cap to store the solvent directly.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-24126 size-full\" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2025\/05\/VFC_47\u7d44\u5408\u5716_EN.jpg\" alt=\"\" width=\"1920\" height=\"1080\" srcset=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2025\/05\/VFC_47\u7d44\u5408\u5716_EN.jpg 1920w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2025\/05\/VFC_47\u7d44\u5408\u5716_EN-768x432.jpg 768w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2025\/05\/VFC_47\u7d44\u5408\u5716_EN-1536x864.jpg 1536w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2025\/05\/VFC_47\u7d44\u5408\u5716_EN-600x338.jpg 600w\" sizes=\"(max-width: 1920px) 100vw, 1920px\" \/><\/p>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><strong><span style=\"font-size: 20px;\"><a id=\"A6\"><\/a>How to Choose a Membrane Filter?<\/span><\/strong><\/h2>\n<p>&nbsp;<\/p>\n<p>Selecting the appropriate membrane is key to ensuring effective filtration. Choices should be based on the specific HPLC system and application:<\/p>\n<p>&nbsp;<\/p>\n<table style=\"border-collapse: collapse; width: 908px;\">\n<tbody>\n<tr>\n<td style=\"width: 151.334px;\"><strong>Pore Size<\/strong><\/td>\n<td style=\"width: 151.334px;\" colspan=\"2\"><strong>Purpose<\/strong><\/td>\n<td style=\"width: 302.666px;\"><strong>Common Materials<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 151.334px;\">0.45 \u03bcm<\/td>\n<td style=\"width: 151.334px;\" colspan=\"2\">General HPLC mobile phase filtration<\/td>\n<td style=\"width: 302.666px;\">PTFE, Nylon<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 151.334px;\">0.2 \u03bcm<\/td>\n<td style=\"width: 151.334px;\" colspan=\"2\">Ultra High Performance Liquid Chromatography (UPLC \/ UHPLC) or sterilization<\/td>\n<td style=\"width: 302.666px;\">PVDF, PTFE<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>Membrane material should also be selected based on the chemical properties of the solvent. Common materials include PTFE, PVDF, Nylon and so on.<br \/>\nBelow are membrane types referenced in methods published or recommended by the Taiwan Food and Drug Administration\u00a0(TFDA):<\/p>\n<p>&nbsp;<\/p>\n<table style=\"border-collapse: collapse; width: 908px;\">\n<tbody>\n<tr>\n<td style=\"width: 302.666px; text-align: center;\" rowspan=\"2\"><strong>Title<\/strong><\/td>\n<td style=\"width: 302.666px; text-align: center;\" rowspan=\"2\"><strong>Author (s)<\/strong><\/td>\n<td style=\"width: 151.334px; text-align: center;\" colspan=\"2\"><strong>Membrane filter<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 151.334px; text-align: center;\" colspan=\"2\">Pore Size<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 302.666px; text-align: center;\"><a href=\"http:\/\/www.apajournal.org.uk\/Vol_32_Part_3.pdf\">DETERMINATION OF PRESERVATIVES IN FOODSTUFFS<\/a><\/td>\n<td style=\"width: 302.666px; text-align: center;\">P. Willetts et.al., 1996<\/td>\n<td style=\"width: 151.334px; text-align: center;\" colspan=\"2\">0.45 \u03bcm<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 302.666px; text-align: center;\"><a href=\"https:\/\/ift.onlinelibrary.wiley.com\/doi\/abs\/10.1111\/j.1365-2621.1986.tb13875.x\">HPLC Method for Determination of inositol Tri-, Tetra-, Penta-, and Hexaphosphates in Foods and Intestinal Contents<\/a><\/td>\n<td style=\"width: 302.666px; text-align: center;\">A.-S. SANDBERG, <br \/>\nR. AHDERINNE, 1986<\/td>\n<td style=\"width: 151.334px; text-align: center;\" colspan=\"2\">0.45 \u03bcm<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 302.666px; text-align: center;\"><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0021967303014614\">Optimization of a new mobile phase to know the complex and real polyphenolic composition: towards a total phenolic index using high-performance liquid chromatography<\/a><\/td>\n<td style=\"width: 302.666px; text-align: center;\">Tsao, R., &amp; Yang, R., 2003<\/td>\n<td style=\"width: 151.334px; text-align: center;\" colspan=\"2\">0.2 \u03bcm \/ 0.45 \u03bcm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-size: 20px;\"><strong>Applications<\/strong><\/span><\/h2>\n<p>&nbsp;<\/p>\n<ul style=\"list-style-type: square;\">\n<li>HPLC mobile phase filtration<\/li>\n<li>Solvent purification and degassing<\/li>\n<li>Removal of microorganisms and contaminants<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-size: 20px;\"><strong>References<\/strong><\/span><\/h2>\n<p>&nbsp;<\/p>\n<ul>\n<li><a href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/knowledge-center\/Mobile-phase-filtration?srsltid=AfmBOopfD4RKKycEoJ8cHdJymzP6qdR-sjuhFIi65x0Nc_Rd7E4zh-j0\">Mobile phase filtration<\/a><\/li>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/TW\/en\/technical-documents\/technical-article\/analytical-chemistry\/small-molecule-hplc\/hplc-filtration-performance-optimizing\">The Importance of Filtration in Optimizing (U)HPLC and LC-MS Performance<\/a><\/li>\n<li><a title=\"Top Tips for LC-MS Sample Preparation\" href=\"https:\/\/www.selectscience.net\/article\/top-tips-for-lc-ms-sample-preparation\">Top Tips for LC-MS Sample Preparation<\/a><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>HPLC Mobile Phase Filtration: The Key Step to Enhance Chromatographic Efficiency &nbsp; &nbsp; Table of Contents &nbsp; What is HPLC? Why Does HPLC Mobile Phase Need Filtration? Why Does Filtration Improve HPLC Stability? How to Perform HPLC Mobile Phase Filtration? ROCKER Complete Solvent Purification Solution How to Choose a Membrane Filter? &nbsp; &nbsp; What is [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":24118,"parent":0,"template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"application-category":[],"class_list":["post-24134","application","type-application","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>HPLC | The Key Step to Enhance Chromatographic Efficiency<\/title>\n<meta name=\"description\" content=\"High Performance Liquid Chromatography (HPLC) is an efficient technique for separation and analysis,capable of separating mixtures and quantitatively analyzing the proportion of each component. 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HPLC is widely used in various scientific fields,including pharmaceuticals, food, cosmetics, health supplements, narcotics, forensic science, and environmental matrices.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.rocker.com.tw\/en\/application\/hplc\/","og_locale":"en_US","og_type":"article","og_title":"HPLC | The Key Step to Enhance Chromatographic Efficiency","og_description":"High Performance Liquid Chromatography (HPLC) is an efficient technique for separation and analysis,capable of separating mixtures and quantitatively analyzing the proportion of each component. 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