{"id":5377,"date":"2020-06-29T01:28:25","date_gmt":"2020-06-29T01:28:25","guid":{"rendered":"http:\/\/rocker.designhu-demo.com\/application\/microbiological_test\/"},"modified":"2026-03-09T11:20:06","modified_gmt":"2026-03-09T03:20:06","slug":"microbiological_test","status":"publish","type":"application","link":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/","title":{"rendered":"Microbiology Test"},"content":{"rendered":"<p>Microbiology test is a vital component of scientific research, clinical diagnostics, and public health. Microbiological testing focuses on the study of microorganisms such as bacteria, viruses, fungi, and parasites, and their impact on human health and the environment.<\/p>\n<p>Understanding the importance of microbiology testing is essential as it enables the identification, characterization, and monitoring of microbiological, allowing for the diagnosis and treatment of infectious diseases, the detection of microbiological food testing, and the assessment of water and air quality.<\/p>\n<p>By discussing microbiology testing, we delve into the methodologies, advancements, and applications.\u00a0<\/p>\n<blockquote><p><!-- Begin Mailchimp Signup Form --><\/p>\n<div id=\"mc_embed_signup\">\n<form id=\"mc-embedded-subscribe-form\" class=\"validate\" action=\"https:\/\/rocker.us12.list-manage.com\/subscribe\/post?u=58ef36f6c5073517cb6c76cc7&amp;id=454e696053&amp;f_id=0072bee0f0\" method=\"post\" name=\"mc-embedded-subscribe-form\" target=\"_self\">\n<div id=\"mc_embed_signup_scroll\">\n<h3>Subscribe for firsthand information!<\/h3>\n<p>&nbsp;<\/p>\n<div class=\"mc-field-group\"><label for=\"mce-EMAIL\">Email<span class=\"asterisk\">:<\/span> <\/label> <input id=\"mce-EMAIL\" class=\"required email\" name=\"EMAIL\" required=\"\" type=\"email\" value=\"\" \/> <span id=\"mce-EMAIL-HELPERTEXT\" class=\"helper_text\"><\/span> <input id=\"mc-embedded-subscribe\" class=\"button\" name=\"subscribe\" type=\"submit\" value=\"Subscribe\" \/><\/div>\n<div id=\"mce-responses\" class=\"clear\">\n<div id=\"mce-error-response\" class=\"response\" style=\"display: none;\">\u00a0<\/div>\n<div id=\"mce-success-response\" class=\"response\" style=\"display: none;\">\u00a0<\/div>\n<\/div>\n<p><!-- real people should not fill this in and expect good things - do not remove this or risk form bot signups--><\/p>\n<div style=\"position: absolute; left: -5000px;\" aria-hidden=\"true\"><input tabindex=\"-1\" name=\"b_58ef36f6c5073517cb6c76cc7_454e696053\" type=\"text\" value=\"\" \/><\/div>\n<\/div>\n<\/form>\n<\/div>\n<p><!--End mc_embed_signup--><\/p><\/blockquote>\n<p>&nbsp;<\/p>\n<div style=\"background-color: #e9eef0; padding: 10px 20px;\"><span style=\"font-size: 20px;\"><strong>Content<\/strong><\/span><br \/>\n<a href=\"#P1\">What is microbiology test?<\/a><br \/>\n<a href=\"#P2\">Common microbiology testing methods<\/a> <br \/>\n<a href=\"#P3\">Microbiology test procedures<\/a> <br \/>\n<a href=\"#P4\">Selecting microbial testing equipment<\/a><br \/>\n<a style=\"font-size: 16px; background-color: #e9eef0;\" href=\"#P5\">Frequently Asked Questions (FAQ)<\/a><\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h2><a id=\"P1\"><\/a>What is microbiology test?<\/h2>\n<p>&nbsp;<\/p>\n<p>Microorganisms are ubiquitous in the natural environment, and they have been widely used in food manufacturing, such as cheese, beer, bread, and nutritional supplements.<\/p>\n<p>However, certain microorganisms are pathogens, such as E. coli, Salmonella, and Staphylococcus aureus, which can cause discomfort and even fetal harm to the human body. Therefore, microbiology testing and microbial detection are essential for quality control in pharmaceutical manufacturing, food and beverage, and cosmetics industries to ensure product safety.<\/p>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><a id=\"P2\"><\/a>Common microbiology testing methods<\/h2>\n<p>&nbsp;<\/p>\n<p>The common methods used for microbiology testing analysis include the multiple-tube fermentation (MPN) method, spread plate method, pour plate method, and membrane filtration method. The membrane filtration method is widely used for the separation of coliform from liquids and has been acknowledged by the U.S. EPA since 1961.<\/p>\n<p>Compared to other methods, the membrane filtration method requires less preparation and provides lower false results and more accurate outcomes. It is considered the primary technology for microorganism separation.<\/p>\n<p>&nbsp;<\/p>\n<p><iframe title=\"YouTube video player\" src=\"https:\/\/www.youtube.com\/embed\/gsXpZlsblFQ\" width=\"560\" height=\"315\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<h3>Comparison of different microbial test methods<\/h3>\n<p>&nbsp;<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"9%\">\n<p>&nbsp;<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Membrane Filtration<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Multiple-tube Fermentation<\/p>\n<\/td>\n<td width=\"21%\">\n<p>Spread Plate<\/p>\n<\/td>\n<td width=\"23%\">\n<p>Pour Plate<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Preparation<\/p>\n<\/td>\n<td width=\"22%\">\n<p>\u00a0Agar plate<\/p>\n<\/td>\n<td width=\"22%\">\n<p>\u00a0Nutrient broth<\/p>\n<\/td>\n<td width=\"21%\">\n<p>Agar plate<\/p>\n<\/td>\n<td width=\"23%\">\n<p>Agar broth (maintained at between 45~50<sup>o<\/sup>C)<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Incubation<\/p>\n<\/td>\n<td width=\"22%\">\n<p>24 \u00b1 2\u00a0 hour<\/p>\n<\/td>\n<td width=\"22%\">\n<p>3 phases, 48 hours\/phase<\/p>\n<\/td>\n<td width=\"21%\">\n<p>48 \u00b1 3 hours<\/p>\n<\/td>\n<td width=\"23%\">\n<p>48 \u00b1 3 hours<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Consumption<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Membrane, petri dish<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Vials and fermentation tubes<\/p>\n<\/td>\n<td width=\"21%\">\n<p>Petri dish, inoculation hoop<\/p>\n<\/td>\n<td width=\"23%\">\n<p>Petri dish<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Sample volume<\/p>\n<\/td>\n<td width=\"22%\">\n<p>High<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Low<\/p>\n<\/td>\n<td width=\"21%\">\n<p>Low<\/p>\n<\/td>\n<td width=\"23%\">\n<p>Low<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Heat-sensitive-bacteria-safe<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Yes<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Yes<\/p>\n<\/td>\n<td width=\"21%\">\n<p>Yes<\/p>\n<\/td>\n<td width=\"23%\">\n<p>No<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Sample selection<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Aqueous sample with low turbidity<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Aqueous sample<\/p>\n<\/td>\n<td width=\"21%\">\n<p>Aqueous sample with high bacteria concentration<\/p>\n<\/td>\n<td width=\"23%\">\n<p>Aqueous sample with low bacteria concentration<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Accuracy<\/p>\n<\/td>\n<td width=\"22%\">\n<p>High<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Very low<\/p>\n<\/td>\n<td width=\"21%\">\n<p>Low<\/p>\n<\/td>\n<td width=\"23%\">\n<p>Low<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Determination<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Observation and counting<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Estimation by MPN<\/p>\n<\/td>\n<td width=\"21%\">\n<p>Observation and counting<\/p>\n<\/td>\n<td width=\"23%\">\n<p>Observation and counting<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Colony splitting<\/p>\n<\/td>\n<td width=\"22%\">\n<p>Yes<\/p>\n<\/td>\n<td width=\"22%\">\n<p>No<\/p>\n<\/td>\n<td width=\"21%\">\n<p>Yes<\/p>\n<\/td>\n<td width=\"23%\">\n<p>Yes<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"9%\">\n<p>Other<\/p>\n<\/td>\n<td width=\"22%\">\n<p>\u2022 Membranes can be moved to different culture medium for incubation.<\/p>\n<p>\u2022 Biocide can be effectively removed.<\/p>\n<\/td>\n<td width=\"22%\">\n<p>\u2022 Able to test aqueous samples with high turbidity<\/p>\n<p>\u2022 Creates more wastes.<\/p>\n<\/td>\n<td width=\"21%\">\n<p>\u2022 Culture medium can be easily scratched by the hoop.<\/p>\n<p>\u2022 Incubation can be processed after dried.<\/p>\n<\/td>\n<td width=\"23%\">\n<p>\u2022 Colonies may exist within the culture medium, more difficult to read.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><a id=\"P3\"><\/a>Microbiology test procedures<\/h2>\n<p>&nbsp;<\/p>\n<p>Procedure of membrane filter method (NIEA E230.55B):<\/p>\n<ul>\n<li>The test should be carried out in a laminar flow hood.<\/li>\n<li>Preparation: Sterilize the stainless steel filtration funnel and tweezers with flame and wait till cool-down.<\/li>\n<\/ul>\n<blockquote>\n<table class=\"no-border\">\n<tbody>\n<tr>\n<td rowspan=\"2\" width=\"60%\">\n<p>Rocker&#8217;s Bunsen burners provide stable flame up to 1300\u2103, are the best choice for microbiological food testing!<br \/>\n&#8211; <a href=\"https:\/\/www.rocker.com.tw\/en\/product\/food-analysis\/lab-burner\/dragon-100-lab-burner\/\">Dragon 100 Torch<\/a> <br \/>\n&#8211; <a href=\"https:\/\/www.rocker.com.tw\/en\/product\/food-analysis\/lab-burner\/dragon-200-lab-burner\/\">Dragon 200 Bunsen Burner<\/a><br \/>\n&#8211; <a href=\"https:\/\/www.rocker.com.tw\/en\/product\/food-analysis\/lab-burner\/dragon-220-lab-burner\/\">Dragon 220 Bunsen Burner (45-degree tilt)<\/a><\/p>\n<\/td>\n<td width=\"40%\">\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2021\/11\/EDM_Dragon.jpg\" alt=\"a combination of different types of common burners that can be found in laboratories, including a torch and 2 Bunsen burners.\" width=\"500\" height=\"383\" \/><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><a href=\"https:\/\/www.rocker.com.tw\/en\/product-category\/food-analysis\/lab-burner\/\">Rocker&#8217;s Dragon Bunsen Burners<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/blockquote>\n<p>&nbsp;<\/p>\n<p>Test procedure:<\/p>\n<ol>\n<li>Place the sterile membrane filter on the filter holder and dilute it with a few drops of diluent to test if the filtration equipment is properly settled.<\/li>\n<li>Shake the samples more than 25 times to homogenize them before dilution and detection.<\/li>\n<li>Filter 100 mL of the samples and then rinse the funnel with 20 mL of diluent.<\/li>\n<li>Place the membrane onto a petri dish with the appropriate culture medium. Ensure that the membrane is firmly attached to the medium, avoiding any air entering in between.<\/li>\n<li>Invert the petri dish and place it in the incubator, incubating at 35 \u00b1 1 oC for 24 \u00b1 2 hours. Use a colony counter to count the number of targeted colonies. Note &#8220;TNTC&#8221; (Too numerous to count) if there are too many colonies.<\/li>\n<li>Determination: Calculate the bacterial density using the formula noted below: (Colony forming units\/100 mL)<br \/>\n<strong>CFU formula(CFU equation):<\/strong><\/li>\n<\/ol>\n<p><img decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2022\/07\/\u6ffe\u819c\u6cd5_\u5b57\u5361_\u7d50\u679c\u8655\u7406En.png\" alt=\"cfu formula and cfu calculation for microbiology analysis\" width=\"1920\" height=\"1080\" \/><\/p>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<h2><a id=\"P4\"><\/a>Selecting microbial testing equipment<\/h2>\n<p>&nbsp;<\/p>\n<p>Microbiology testing, particularly with membrane filtration, often requires sample replication, liquid dispensing, and generates a large amount of liquid waste. Proper waste handling, accurate sample preparation, and equipment sterilization are essential but can be tedious. Here, we share some key points for you to consider when deciding on equipment to simplify your lab life.<\/p>\n<p>&nbsp;<\/p>\n<h3><strong>1) Choose equipment that can be sterilized rapidly.<\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p>\nMicrobiology testing equipment often requires sterilization using an autoclave, which can take hours to complete. To expedite the process and ensure smoother workflow, consider preparing several sets of equipment for replacement. Alternatively, you can opt for materials that can be rapidly sterilized, such as stainless steel, which can be flame sanitized and ready for use once it has cooled down. This approach saves significant time and facilitates a smoother work procedure, particularly when dealing with a large number of samples to test.<\/p>\n<blockquote>\n<table class=\"no-border\">\n<tbody>\n<tr>\n<td style=\"vertical-align: middle;\" rowspan=\"2\" width=\"60%\">\n<p><a href=\"https:\/\/www.rocker.com.tw\/en\/product\/laboratory-filtration-system\/filter-holder\/sf-stainless-steel-filter-holder\/\">Rocker SF<\/a> Stainless Steel Filter Holder<br \/>\n&#8211; Spin-lock design, no clamps required.<br \/>\n&#8211; SS316 &#8211; made safe &amp; heat resistant<br \/>\n&#8211; Cost-effective, saves up to 46% of long-term budget.\u00a0<br \/>\n&#8211; Eco-friendly, no plastics.<br \/>\n<a href=\"\/?product_cat=multi-branch-vacuum-manifold\">Rocker Stainless Steel Manifold<\/a><br \/>\n<a href=\"\/?product_cat=multivac-vacuum-filtration-system\">Rocker Vacuum Manifol Systems<\/a><\/p>\n<\/td>\n<td width=\"40%\">\n<p><img decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2020\/08\/MultiVac601_MB_01.jpg\" alt=\"vacuum filtration manifold made of steel\" width=\"500\" height=\"383\" \/><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><a href=\"\/?product_cat=multivac-vacuum-filtration-system\">Stainless Manifold and Filter Holders<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/blockquote>\n<p>&nbsp;<\/p>\n<h3><strong>2) Easier liquid draining<\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p>\nLiquid waste generated from microbiology tests of water samples does not require additional treatment before being drained into the sink. Therefore, there is no need for liquid collection before draining it. Choosing equipment that can directly drain the liquid waste into the sink can save you both effort and time.<\/p>\n<blockquote>\n<table class=\"no-border\">\n<tbody>\n<tr>\n<td style=\"vertical-align: middle;\" rowspan=\"2\" width=\"60%\">\n<p><a href=\"\/?product_cat=watervac-filtration-system-for-liquid-transfer\">Rocker WaterVac Filtration System<\/a><br \/>\n&#8211; <a href=\"https:\/\/www.rocker.com.tw\/en\/product\/laboratory-pump-en\/diaphragm-liquid-pump\/alligator-200-diaphragm-liquid-pump\/\">Alligator 200<\/a>: Taiwan Excellence award-winning pump<br \/>\n&#8211; <a href=\"https:\/\/www.rocker.com.tw\/en\/product\/laboratory-filtration-system\/filtration-system-en\/watervac-100-mb-filtration-system\/\">WaterVac 100<\/a>: 24VDC-driven, easily portable for at-scene sample collection.<br \/>\n&#8211; <a href=\"https:\/\/www.rocker.com.tw\/en\/product\/laboratory-filtration-system\/filtration-system-en\/watervac-201-mb-filtration-system\/\">WaterVac 200<\/a>: 2 samples at once. Space-saver.<\/p>\n<\/td>\n<td width=\"40%\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2020\/08\/WaterVac201_MB_01.jpg\" alt=\"all-in-one vacuum filtration system that has 2 metal (stainless steel) funnels on top of it, which integrates pump, piping and filtration funnels\" width=\"500\" height=\"383\" \/><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><a href=\"https:\/\/www.rocker.com.tw\/en\/product\/laboratory-filtration-system\/filtration-system-en\/watervac-201-mb-filtration-system\/\">Rocker WaterVac 200 Direct Drain Filtration System\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/blockquote>\n<h3><strong>3) Improve sample dispensing efficiency and consistency<\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p>\nWhen handling a large number of samples, efficient and precise liquid dispensing becomes critical. We recommend using a dispensing system like the <strong data-start=\"1710\" data-end=\"1730\">Buller 600 \u2013 DSP<\/strong>, which features preset volume settings (such as 2.5 mL, 5 mL, and 9 mL) and an ergonomic <span data-teams=\"true\">handling<\/span> dispenser. This design not only streamlines operation but also helps maintain dispensing accuracy and consistency, making it particularly suitable for standardized microbiological testing workflows.<\/p>\n<blockquote>\n<table class=\"no-border\">\n<tbody>\n<tr>\n<td style=\"vertical-align: middle;\" rowspan=\"2\" width=\"60%\">\n<p><a href=\"https:\/\/www.rocker.com.tw\/en\/product\/food-analysis\/peristaltic_pump-dsp\/buller-600-dsp-dispensing-peristaltic-pump\/\">Buller 600 \u2013 DSP Dispensing Peristaltic Pump<\/a><br \/>\n&#8211; Design for microbiological test<br \/>\n&#8211; Ergonomic &amp; comfortable<br \/>\n&#8211; Accurate dispensing<\/p>\n<\/td>\n<td width=\"40%\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2024\/06\/Buller-600_DSP-scaled.jpg\" alt=\"\" width=\"500\" height=\"383\" \/><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><a href=\"https:\/\/www.rocker.com.tw\/en\/product\/food-analysis\/peristaltic_pump-dsp\/buller-600-dsp-dispensing-peristaltic-pump\/\">Buller 600 \u2013 DSP Dispensing Peristaltic Pump<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/blockquote>\n<figure id=\"attachment_29055\" aria-describedby=\"caption-attachment-29055\" style=\"width: 900px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.rocker.com.tw\/en\/contact\/\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-29055 size-full\" src=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2023\/04\/rocker-scientific-global-dealer-network-quick-quote-1.webp-1.webp\" alt=\"Rocker Global Dealer Network banner offering dedicated support from local experts in USA, India, China, Indonesia, Malaysia, and worldwide for quick quotes.\" width=\"900\" height=\"120\" srcset=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2023\/04\/rocker-scientific-global-dealer-network-quick-quote-1.webp-1.webp 900w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2023\/04\/rocker-scientific-global-dealer-network-quick-quote-1.webp-1-768x102.webp 768w, https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2023\/04\/rocker-scientific-global-dealer-network-quick-quote-1.webp-1-600x80.webp 600w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/a><figcaption id=\"caption-attachment-29055\" class=\"wp-caption-text\">Global expert support across USA, Asia, and worldwide. \u25b2Click to get a quick quote from your local Rocker dealer.<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>References:<\/strong><\/p>\n<p>&#8211;<a href=\"https:\/\/www.pall.com\/en\/laboratory\/blog\/microbial-qc-testing.html\">Pour Plate and Most Probable Number tests have changed little in 50 years, while new technology has advanced Membrane Filtration Tests for microbial QC<\/a><\/p>\n<p>&#8211;<a href=\"https:\/\/nepis.epa.gov\/Exe\/ZyPDF.cgi?Dockey=P100WD73.txt\">Analytical Methods Approved for Compliance Monitoring under the Revised Total Coliform Rule, EPA 815-B-19-007, EPA, 2019<\/a><\/p>\n<p>&#8211;<a href=\"https:\/\/www.differencebetween.com\/difference-between-pour-plate-and-spread-plate\/#Pour%20Plate%20vs%20Spread%20Plate%20in%20Tabular%20Form\">Difference Between Pour Plate and Spread Plate<\/a><\/p>\n<p>&#8211;<a href=\"https:\/\/www.fda.gov\/files\/food\/published\/Equivalent-Testing-Methodologies-for-Agricultural-Water-%28PDF%29.pdf\">EQUIVALENT TESTING METHODOLOGY FOR AGRICULTURAL WATER, FDA<\/a><\/p>\n<p>&#8211;<a href=\"https:\/\/www.pharmacy180.com\/article\/enumeration-of-microorganisms-372\/\">ENUMERATION OF MICROORGANISMS<\/a><\/p>\n<p>&#8211;<a href=\"https:\/\/www.researchgate.net\/publication\/328569551_Microbial_Aspects_of_Drinking_Water\">Microbial Aspects of Drinking Water<\/a><\/p>\n<p>&nbsp;<\/p>\n<hr \/>\n<h2>\u00a0<\/h2>\n<h2><strong><span style=\"font-size: 24px;\"><a id=\"P5\"><\/a>Frequently Asked Questions (FAQ)<\/span><\/strong><\/h2>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000080; font-size: 16px;\"><strong>Q1: Why is membrane filtration <span class=\"TextRun Highlight SCXW33605196 BCX0\" lang=\"EN-US\" xml:lang=\"EN-US\" data-contrast=\"none\"><span class=\"NormalTextRun SCXW33605196 BCX0\">widely used<\/span><\/span><span class=\"TextRun SCXW33605196 BCX0\" lang=\"ZH-TW\" xml:lang=\"ZH-TW\" data-contrast=\"none\"><span class=\"NormalTextRun SCXW33605196 BCX0\">\u00a0<\/span><\/span><span class=\"TextRun SCXW33605196 BCX0\" lang=\"EN-US\" xml:lang=\"EN-US\" data-contrast=\"auto\"><span class=\"NormalTextRun SCXW33605196 BCX0\">for bioburden testing<\/span><\/span><span class=\"TextRun SCXW33605196 BCX0\" lang=\"ZH-TW\" xml:lang=\"ZH-TW\" data-contrast=\"auto\"><span class=\"NormalTextRun SCXW33605196 BCX0\">\u00a0<\/span><\/span><span class=\"TextRun Highlight SCXW33605196 BCX0\" lang=\"EN-US\" xml:lang=\"EN-US\" data-contrast=\"none\"><span class=\"NormalTextRun SCXW33605196 BCX0\">of liquid samples<\/span><\/span>?<\/strong><\/span><\/h3>\n<p>&nbsp;<\/p>\n<p><strong>A1: <\/strong>Membrane filtration is preferred because it can concentrate microorganisms from large volumes of liquid samples. By using a 0.45\u03bcm pore size membrane, it effectively separates microbes from potential inhibitors (like antibiotics or preservatives) in the sample, ensuring more accurate colony counting and compliance with pharmacopeia standards.<\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000080; font-size: 16px;\"><strong>Q2:<\/strong> How can I improve the efficiency of high-throughput microbiological testing?<\/span><\/h3>\n<p>&nbsp;<\/p>\n<p><strong>A2:<\/strong> The most effective way is to use a <b data-path-to-node=\"7\" data-index-in-node=\"38\">multi-branch manifold<\/b> (e.g., MultiVac 601-MB) combined with a <b data-path-to-node=\"7\" data-index-in-node=\"100\">diaphragm liquid pump<\/b>. This setup allows simultaneous filtration of multiple samples and eliminates the need to stop and empty waste bottles, reducing total processing time by up to 40%.<\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000080; font-size: 16px;\"><strong>Q3:<\/strong> What are the advantages of using the Alligator 200 Liquid Pump in a microbiology lab?<\/span><\/h3>\n<p>&nbsp;<\/p>\n<p><strong>A3:<\/strong> Unlike traditional vacuum pumps, the\u202fAlligator 200\u202ftransfers filtered liquid directly to the drain. This \u201cbottle-free\u201d design saves significant bench space, reduces the need to interrupt workflows to empty waste bottles, and prevents accidental overflow. It supports a cleaner and more streamlined laboratory environment.<\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000080; font-size: 16px;\"><strong>Q4:<\/strong> How does a dispensing peristaltic pump assist in microbial analysis?<\/span><\/h3>\n<p>&nbsp;<\/p>\n<p><strong>A4:<\/strong> A dispensing pump like the <b data-path-to-node=\"11\" data-index-in-node=\"30\">Buller 600 DSP<\/b> is essential for the precise filling of culture media or diluents. It automates repetitive liquid handling with high accuracy, reducing manual pipetting errors and maintaining a sterile workflow during sample preparation<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Microbiology test is a vital component of scientific research, clinical diagnostics, and public health. Microbiological testing focuses on the study of microorganisms such as bacteria, viruses, fungi, and parasites, and their impact on human health and the environment. Understanding the importance of microbiology testing is essential as it enables the identification, characterization, and monitoring of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":4900,"parent":0,"template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"application-category":[],"class_list":["post-5377","application","type-application","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Microbiological Testing Guide | Tips to Make It Quick &amp; Easy<\/title>\n<meta name=\"description\" content=\"Learn key microbiology test methods, CFU formulas, and equipment selection tips for accurate microbial testing in labs\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Microbiological Testing Guide | Tips to Make It Quick &amp; Easy\" \/>\n<meta property=\"og:description\" content=\"Learn key microbiology test methods, CFU formulas, and equipment selection tips for accurate microbial testing in labs\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/\" \/>\n<meta property=\"og:site_name\" content=\"Rocker\" \/>\n<meta property=\"article:modified_time\" content=\"2026-03-09T03:20:06+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2020\/06\/icon_\u5fae\u751f\u7269\u6aa2\u6e2c.png\" \/>\n\t<meta property=\"og:image:width\" content=\"250\" \/>\n\t<meta property=\"og:image:height\" content=\"250\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"6 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Microbiological Testing Guide | Tips to Make It Quick & Easy","description":"Learn key microbiology test methods, CFU formulas, and equipment selection tips for accurate microbial testing in labs","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/","og_locale":"en_US","og_type":"article","og_title":"Microbiological Testing Guide | Tips to Make It Quick & Easy","og_description":"Learn key microbiology test methods, CFU formulas, and equipment selection tips for accurate microbial testing in labs","og_url":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/","og_site_name":"Rocker","article_modified_time":"2026-03-09T03:20:06+00:00","og_image":[{"width":250,"height":250,"url":"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2020\/06\/icon_\u5fae\u751f\u7269\u6aa2\u6e2c.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"6 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/","url":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/","name":"Microbiological Testing Guide | Tips to Make It Quick & Easy","isPartOf":{"@id":"https:\/\/www.rocker.com.tw\/en\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/#primaryimage"},"image":{"@id":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/#primaryimage"},"thumbnailUrl":"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2020\/06\/icon_\u5fae\u751f\u7269\u6aa2\u6e2c.png","datePublished":"2020-06-29T01:28:25+00:00","dateModified":"2026-03-09T03:20:06+00:00","description":"Learn key microbiology test methods, CFU formulas, and equipment selection tips for accurate microbial testing in labs","breadcrumb":{"@id":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/#primaryimage","url":"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2020\/06\/icon_\u5fae\u751f\u7269\u6aa2\u6e2c.png","contentUrl":"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2020\/06\/icon_\u5fae\u751f\u7269\u6aa2\u6e2c.png","width":250,"height":250,"caption":"Microbiological Test"},{"@type":"BreadcrumbList","@id":"https:\/\/www.rocker.com.tw\/en\/application\/microbiological_test\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.rocker.com.tw\/en\/"},{"@type":"ListItem","position":2,"name":"Applications","item":"https:\/\/www.rocker.com.tw\/en\/application\/"},{"@type":"ListItem","position":3,"name":"Microbiology Test"}]},{"@type":"WebSite","@id":"https:\/\/www.rocker.com.tw\/en\/#website","url":"https:\/\/www.rocker.com.tw\/en\/","name":"Rocker","description":"Rocker - make filtration easy!","publisher":{"@id":"https:\/\/www.rocker.com.tw\/en\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.rocker.com.tw\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.rocker.com.tw\/en\/#organization","name":"Rocker","url":"https:\/\/www.rocker.com.tw\/en\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.rocker.com.tw\/en\/#\/schema\/logo\/image\/","url":"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2020\/09\/email-logo-1.png","contentUrl":"https:\/\/www.rocker.com.tw\/wp-content\/uploads\/2020\/09\/email-logo-1.png","width":500,"height":91,"caption":"Rocker"},"image":{"@id":"https:\/\/www.rocker.com.tw\/en\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.linkedin.com\/company\/rocker-scientific","https:\/\/www.youtube.com\/c\/RockerScientific\/featured"]}]}},"_links":{"self":[{"href":"https:\/\/www.rocker.com.tw\/en\/wp-json\/wp\/v2\/application\/5377","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.rocker.com.tw\/en\/wp-json\/wp\/v2\/application"}],"about":[{"href":"https:\/\/www.rocker.com.tw\/en\/wp-json\/wp\/v2\/types\/application"}],"author":[{"embeddable":true,"href":"https:\/\/www.rocker.com.tw\/en\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":8,"href":"https:\/\/www.rocker.com.tw\/en\/wp-json\/wp\/v2\/application\/5377\/revisions"}],"predecessor-version":[{"id":29078,"href":"https:\/\/www.rocker.com.tw\/en\/wp-json\/wp\/v2\/application\/5377\/revisions\/29078"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.rocker.com.tw\/en\/wp-json\/wp\/v2\/media\/4900"}],"wp:attachment":[{"href":"https:\/\/www.rocker.com.tw\/en\/wp-json\/wp\/v2\/media?parent=5377"}],"wp:term":[{"taxonomy":"application-category","embeddable":true,"href":"https:\/\/www.rocker.com.tw\/en\/wp-json\/wp\/v2\/application-category?post=5377"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}